DNA gyrase structure. A Schematic of DNA gyrase bound to DNA prior to
What Is Puc19. Web puc19 sequences (3) addgene sequences: Web puc19 is a commonly used plasmid cloning vector in e.
Coli vector with a multiple cloning site (mcs) for dna cloning. Puc19 this plasmid vector also contains important features. Academic & science » universities. The early work on this plasmid series was conducted at the university of california. Add 1 µl (10 ng) puc19 control dna to one tube. It is a small plasmid with a high copy number. Web thermo scientific puc19 vector is a small, high copy number, e. It contains the lacz gene and has multiple cloning sites. Governmental » state & local. Puc19 plasmid is similar to pbr322 plasmid in structure.
The mcs is reversed in puc18. Hence, it is widely used as a cloning vector. Web puc19 sequences (3) addgene sequences: Incubate the cells on ice for 30 minutes; Transfer the cells to 37° c water bath for exactly 45 seconds Mix by gentle tapping and place on ice; Web a puc19 cloning vector showing the multiple cloning site sequence with restriction enzyme sites. The molecular weight of the puc19 vector is 1.75×10 6 da. The mcs is reversed in puc18. Analyze sequence genbank snapgene file help Coli plasmid cloning vector, of which multiple cloning sites as shown below.
