Columns for high mass loading low pH stability & high efficiency Waters
What Is Endcapping In Hplc Column. Web different endcapping procedures are utilized today in modern hplc column phases. Some legacy applications were developed with nonendcapped materials, and so those must be used to reproduce those results.
Columns for high mass loading low pH stability & high efficiency Waters
Retain and resolve polar analytes using thermo scientific™ hypersil gold™ aq polar endcapped c18 columns. [1] endcapped columns show decreased retention for hydrogen bond acceptors, such as ionized bases, and. Web the kind and particle size of the column packing material. Web hss t3 packings can run in 100% aqueous mobile phase and are designed to retain both polar and nonpolar compounds. Endcapping refers to the replacement of available silanol groups in a bonded stationary phase with tms. Depending on the desired application, many hplc columns are available. Some legacy applications were developed with nonendcapped materials, and so those must be used to reproduce those results. Endcapped materials have a higher carbon load on the surface and avoid most of the reactions with silanols that can lead to skewed peaks. Web a c18 column with polar endcapping aqua is a conventional reversed phase hplc media with polar endcapping that provides increased retention of polar small molecules and 100% aqueous stability. These columns provide excellent peak shape, even with challenging reversed phase separations using highly aqueous mobile.
[1] endcapped columns show decreased retention for hydrogen bond acceptors, such as ionized bases, and. Web a c18 column with polar endcapping aqua is a conventional reversed phase hplc media with polar endcapping that provides increased retention of polar small molecules and 100% aqueous stability. Web hss t3 packings can run in 100% aqueous mobile phase and are designed to retain both polar and nonpolar compounds. Different packing materials support other separation mechanisms. Endcapping refers to the replacement of available silanol groups in a bonded stationary phase with tms. Web the kind and particle size of the column packing material. Web hypersil gold™ aq c18 reversed phase hplc column, 5 μm, 4.6 mm x 250 mm thermo scientific™. Some legacy applications were developed with nonendcapped materials, and so those must be used to reproduce those results. Retain and resolve polar analytes using thermo scientific™ hypersil gold™ aq polar endcapped c18 columns. The figure below shows the traditional stationary phase bonding and tms endcapping reaction: For new methods, upgrade to luna omega polar c18 for small molecules or biozen columns for peptides and peptide mapping.
